Coding

Part:BBa_K4780003

Designed by: Jack Dalton   Group: iGEM23_ANU-Australia   (2023-10-02)


This is a synthetic arsenic binding protein

This is a synthetic arsenic binding protein based on ArsR from E. coli designed using protein MPNN. Did not express well in E. coli DE3 cells. We switched to T7 Shuffle cells as they express cysteine rich proteins better and this resulted in strong expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 236
    Illegal XhoI site found at 166
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None